#4592 A HIGH PROTEIN DIET INDUCES DEPRESSION-LIKE BEHAVIOR IN CKD RODENT MODELS

Abstract Background and Aims The prevalence of depression among patients with Chronic Kidney Disease (CKD) is higher than the general population. Around 21-27% CKD estimated to be affected by depression. Several studies hypothesized that mood disorders may due reduced family support, older age, low educational status quality life. However, adults are also known exhibit altered tryptophan metabolism causing plasma levels elevated kynurenine kynurenic acid. Moreover, show increased indoxyl sulfate indole-3-acetic acid, two protein-bound uremic toxins deriving from gut microbiome tryptophan. We postulate not just life but a biological reason. In addition, we hypothesize high protein diet influence therefore affect symptoms. Method Eighteen Sprague-Dawley male rats (Janvier, Le Genest- St Isle, France), 7-8 weeks old, were induced utilizing 5/6 nephrectomy randomly assigned (n = 10) or 8) diet. A sham-operated control group for each was used 7 respectively). splash test adopted assess depressive symptoms in rats. short, behavioral consisting sprinkling 10% sucrose solution on dorsal coat rat. Latency (time before initiation grooming) duration grooming recorded 5 min as an indication self-care behavior. Delayed considered depression-like behavior Blood urine creatinine determined standard laboratory techniques. Total plasmatic concentrations tryptophan, kynurenine, acid measured using LC-MS/MS. Results latency starting significantly (p .0499) compared sham same while no difference seen between groups. reported Interestingly, when administering .0025). Among rats, correlated clearance (Spearman r -.57, p value .019), .64, .007), .63, .009). total time shorter both groups .4881 .4312, Conclusion These results suggest factors contribute onset decline kidney function accumulation potential risk Dietary interventions such diet, strategies lower which alleviate depressive-like